Biology and clinical application of stem cells

The course analyzes the types of stem cells (embryonic, adult and induced pluripotent stem cells) and clinical application. Specific topics include:

  • General aspects of regeneration.
  • Stem cells with embryonic origin: properties of embryonic carcinoma stem cells (EC) and embryonic stem cells (ES); isolation and propagation of embryonic stem cells lines (mouse and human cell lines); differentiation of embryonic stem cells: strategies for directing differentiation of embryonic stem cells into hematopoietic cells, cardiomyocytes, insulin-producing cells, neuronal cells, epithelial cells, skeletal muscle cells, germ cell cells; immunogenicity of embryonic stem cells; in vitro fertilization-source of stem cells; guidelines for human embryonic stem cell research and clinical applications.
  • Adult stem cells: characteristics of stem and progenitor cells; stem cell microenvironment (niche); stem cell migration and plasticity; hematopoietic stem cells (bone marrow; peripheral blood; umbilical cord); mesenchymal stem cells; muscular stem cells (satellite cells); pancreatic stem cells; epidermal stem cells; corneal stem cells ; neural stem cells; liver stem cells; cancer stem cells.
  • Induced pluripotent stem cells (iPSCs).